Virtualbox 32 Bit Host

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  • But, now when I run 'VirtualBox' within this VM, all of those settings appear as above, but when I try to create a VM from this process, only 32-bit options appear. So: A 64-bit (hardware) host runs a 64-bit (virtual) host, which 'refuses' to configure a 64-bit guest.
  • I google for '64 bit guest on 32 bit host' (it's among the suggestions once you start typing 'virtualbox 64') and get lots of: 'Now 64 bit guests supported on 32 bit hosts'. Next, I try to boot, and it gives me: 'your CPU is not 64-bit capable'.

Windows 7 (32-bit and 64-bit) Windows 8 (32-bit and 64-bit) Windows 8.1 (32-bit and 64-bit) Windows 10 RTM build 10240 (32-bit and 64-bit) Windows Server 2012 (64-bit) Windows Server 2012 R2 (64-bit) Windows Server 2016 (64-bit) 1 Support for 64-bit Windows was added with VirtualBox 1.5. Support for Windows XP was removed with VirtualBox 5.0. Creating a New Virtual Machine in VirtualBox. To create a new virtual machine, you need to start VirtualBox. On the host where you installed Oracle VDI and VirtualBox, select the Applications menu on the desktop, then the System Tools menu, and then Oracle VM VirtualBox. Alternatively, you can run the VirtualBox command in a terminal.

Because Oracle VM VirtualBox is designed to provide a generic virtualization environment for x86 systems, it can run operating systems (OSes) of any kind. However, Oracle VM VirtualBox focuses on the following guest systems:

  • Windows NT 4.0:

    • Fully supports all versions, editions, and service packs. Note that you might encounter issues with some older service packs, so install at least service pack 6a.

    • Guest Additions are available with a limited feature set.

  • Windows 2000, Windows XP, Windows Server 2003, Windows Vista, Windows Server 2008, Windows 7, Windows Server 2008 R2, Windows 8, Windows Server 2012, Windows 8.1, Windows Server 2012 R2, Windows 10 (non-Insider Preview releases), Windows Server 2016, Windows Server 2019:

    • Fully supports all versions, editions, and service packs, including 64-bit versions.

    • Note that you must enable hardware virtualization when running at least Windows 8.

    • Guest Additions are available.

  • MS-DOS, Windows 3.x, Windows 95, Windows 98, Windows ME:

    • Limited testing has been performed.

    • Use beyond legacy installation mechanisms is not recommended.

    • Guest Additions are not available.

  • Linux 2.4:

    Limited support.

  • Linux 2.6:

    • Fully supports all versions and editions, both 32-bit and 64-bit.

    • For best performance, use at least Linux kernel version 2.6.13.

    • Guest Additions are available.

    Certain Linux kernel releases have bugs that prevent them from executing in a virtual environment. See Buggy Linux 2.6 Kernel Versions.

  • Linux 3.x and later:

    • Fully supports all versions and editions, both 32-bit and 64-bit.

    • Guest Additions are available.

  • Oracle Solaris 10 and Oracle Solaris 11:

    • Fully supports all versions starting with Oracle Solaris 10 8/08 and Oracle Solaris 11.

    • Supports 64-bit prior to Oracle Solaris 11 11/11, and 32-bit.

    • Guest Additions are available.

  • FreeBSD:

    • Limited support.

    • Note that you must enable hardware virtualization when running FreeBSD.

    • Guest Additions are not available.

  • OpenBSD:

    • Supports at least version 3.7.

    • Note that you must enable hardware virtualization when running OpenBSD.

    • Guest Additions are not available.

  • OS/2 Warp 4.5:

    • Only MCP2 is supported. Other OS/2 versions might not work.

    • Note that you must enable hardware virtualization when running OS/2 Warp 4.5.

    • Guest Additions are available with a limited feature set. See Known Limitations.

  • Mac OS X:

    • Oracle VM VirtualBox 3.2 added experimental support for Mac OS X guests, with restrictions. See Section 3.1.1, “Mac OS X Guests” and Known Limitations.

    • Guest Additions are not available.

Oracle VM VirtualBox enables you to install and execute unmodified versions of Mac OS X guests on supported host hardware. Note that this feature is experimental and thus unsupported.

Oracle VM VirtualBox is the first product to provide the modern PC architecture expected by OS X without requiring any of the modifications used by competing virtualization solutions. For example, some competing solutions perform modifications to the Mac OS X install DVDs, such as a different boot loader and replaced files.

Be aware of the following important issues before you attempt to install a Mac OS X guest:

  • Mac OS X is commercial, licensed software and contains both license and technical restrictions that limit its use to certain hardware and usage scenarios. You must understand and comply with these restrictions.

    In particular, Apple prohibits the installation of most versions of Mac OS X on non-Apple hardware.

    These license restrictions are also enforced on a technical level. Mac OS X verifies that it is running on Apple hardware. Most DVDs that accompany Apple hardware check for the exact model. These restrictions are not circumvented by Oracle VM VirtualBox and continue to apply.

  • Only CPUs that are known and tested by Apple are supported. As a result, if your Intel CPU is newer than the Mac OS X build, or if you have a non-Intel CPU, you will likely encounter a panic during bootup with an 'Unsupported CPU' exception.

    Ensure that you use the Mac OS X DVD that comes with your Apple hardware.

  • The Mac OS X installer expects the hard disk to be partitioned. So, the installer will not offer a partition selection to you. Before you can install the software successfully, start the Disk Utility from the Tools menu and partition the hard disk. Close the Disk Utility and proceed with the installation.

  • In addition, Mac OS X support in Oracle VM VirtualBox is an experimental feature. See Known Limitations.

Be sure to enable I/O APIC for virtual machines that you intend to use in 64-bit mode. This is especially true for 64-bit Windows VMs. See Section 3.4.2, “Advanced Tab”. For 64-bit Windows guests, ensure that the VM uses the Intel networking device because there is no 64-bit driver support for the AMD PCNet card. See Section 6.1, “Virtual Networking Hardware”.

If you use the Create VM wizard of the VirtualBox Manager, Oracle VM VirtualBox automatically uses the correct settings for each selected 64-bit OS type. See Section 1.7, “Creating Your First Virtual Machine”.

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Oracle VirtualBox is a cross-platform virtualization application. It installs on your existing Intel or AMD-based computers, whether they are running Windows, Mac, Linux or Solaris operating systems. VirtualBox can create and run a 'guest' operating system (virtual machine) in a window of the host operating system. The virtual machine provides a self-contained environment in which to experiment with new software without risking damaging changes to the host operating system.

We will give an instruction with step-by-step screenshots to show how to install VirtualBox with an example of installing Ubuntu OS as a guest machine. We also explain how to share files between the host and guest operating systems.

Downloads

Note the instruction here is based on the latest version of the VirtualBox. If you have already installed an earlier version of VirtualBox, your experience may be different. It is recommended to use the latest version of VirtualBox to avoid issues. The VirtualBox forum is a good place to find a solution if you see any problems.

Go to VirtualBox website here to download the binary for your current operating system. Since our host machine is running on Windows, I'll choose 'x86/amd64' from Windows hosts. When download is finished, run the executable file. Continue with the installation of VirtualBox with the defaults. This will open VirtualBox at the end of the installation.

Create Virtual Machine

Click 'New' button to open a dialog.

Type a name for the new virtual machine. Since I am planning to install Ubuntu 14.04, I'll enter 'ubuntu1404'. Note that VirtualBox automatically changes 'Type' to Linux and 'Version' to 'Ubuntu (64 bit)'. These two options are exactly what we need.

The memory size depends on your host machine memory size. In my case, I have 12GB physical RAM. I like to allocate as much as possible for Ubuntu but leave some for my Windows host machine. I pick 8192 MB for my Ubuntu. Note that VirtualBox will create a swap partition with the same amount space as base memeory you have entered here. So later when you are selecting the size of the virtual hard drive, make sure it is large enough since the hard drive will be splitted into root (/)and swap partitions. The root partition contains by default all your system files, program settings and documents.

Accept the default 'Create a virtual hard drive now' and click 'Create' button.

Continue to accept the default 'VDI' drive file type and click 'Next' button.

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Change the storage type from the default 'Dynamically allocated' to 'Fixed size' to increase performance.

For the virtual hard drive space, the default value is 8GB which is too little for RNA-Seq analysis. I'll pick 100GB since I have plenty of space in my hard disk. You want to choose a good size for your RNA-Seq analysis. If you realize the drive space is not large enough, you'll need to go over these steps again to create another virtual machine.

Click 'Create' button and VirtualBox will generate Ubuntu virtual machine.

Now the virtual machine is created. We are ready to install Ubuntu in this virtual machine. Select your new virtual machine and click 'Settings' button. Click on 'Storage' category and then 'Empty' under Controller:IDE. Click 'CD/DVD' icon on right hand side and select the ubuntu ISO file to mount.

Note that if you have not downloaded 64-bit Ubuntu ISO file, you can check out this page for more information. When downloading Ubuntu ISO file, make sure to selecte 64-bit version. Also make sure the VT-x/Virtualization Technology has been enabled in your computer's BIOS/Basic Input Output System.

Since Tophat program can take an advantage of multiple processors/threads, it is a good idea to specify a large number of processors in virtual machine (default value is 1). You can change this number by clicking on 'System' category. In this case, I change the number of CPUs to 4 since 4 is the largest value shown on the green bar in my case. Now you can click 'OK' button to continue.

VirtualBox may pop up a message about 'Auto capture keyboard' option. Read the message there and check 'Do not show this message again' option before clicking OK.

Install Ubuntu

Back to Oracle VM VirtualBox Manager, click on the new Ubuntu virtual machine and hit 'Start' button. Now you shall see a 'Welcome' screen. Click 'Install Ubuntu' button. Note that the installation process may differ a little bit from version to version. The screenshots here are based on Ubuntu 14.04.1.

Click 'Continue' button.

Make sure 'Erase disk and install Ubuntu' option is selected and click 'Install Now' button.

Ubuntu will ask you a few questions. If the default is good, click 'Continue' button.

In 'Who are you?' dialog, enter your preferred name, username and password. Note that this user will have root/sudo privilege. Click 'Continue' button.

The installation will continue until it is finished.

After installation is complete, click 'Restart Now' button. When you see a screen with a black background saying 'Please remove installation media and close the tray (if any) then press ENTER:', just follow it.

Enter the password you have chosen and press 'Enter'.

The Ubuntu Desktop OS is ready. You may find the desktop screen is too small. Don't worry. You can solve this easily with 'VirtualBox Guest Additions'.

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Guest Additions

After the Ubuntu guest machine is installed, you will find the Ubuntu OS screen size is not extendable and you are not able to share folders between the host machine and Ubuntu.

Guest Additions software provide additional capability to a guest virtual machine, including mouse pointer integration, better video support, share folders, share clipboard, et al.

Before we proceed to install Guest Additions, make sure the current user has sudo/root privilege. If the current user does not have sudo/root privilege or it is not sure, run the following terminal command from an account (such as the user created when Ubuntu was first created; see the screenshot of 'Who are you?' above) with root privileges to enable the root privilege for the current user where USERNAME should be replaced by the current user's name.

To install Guest Additions, click Devices > Insert Guest Additions CD images…

Click 'Run' button (next to 'Cancel' button) to start the installation. Note that the version of VBOXADDITIONS should be matched with the one of VirtualBox you have installed.

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Installing Guest Additions requires root privilege. Enter user's password (assume the current user has sudo privilege).

When the installation is finished, press Return key to close the terminal window.

Now prepare to restart the system to enable the change. Click 'Shutdown' on the menu and then the 'Restart' button.

After rebooting Ubuntu, you will see the desktop resolution is much better.

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Share Folders

Before you can enable shared folders on the host machine, make sure you have installed VirtualBox Guest Additions software on the guest machine.

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When Ubuntu guest machine is on, click on Devices > Shared folders settings…

Select Machine Folders and hit the add button (plus sign).

Browse to the folder you wish to access from the guest machine. Check Auto-mount and Make Permanent options and click OK button.

Click OK button once more.

Now type 'terminal' in Dash to open a Terminal.

Type the following line in the Terminal to add a user to 'vboxsf' group. This step is necessary in order to use the VB's 'Shared Folder' feature.Replace 'brb' with your account name in Ubuntu.When you are done, restart the Ubuntu guest machine and go to /media/ directory. From Nautilus (file manager in Ubuntu), click Computer > File System > Media folder and inside it you will see a folder beginning with sf_ (the folder name is sf_Downloads in our example). Now you can transfer files between the Ubuntu guest machine and the Windows host machine in Nautilus.

Although Shared Folder system in VirtualBox is a nice feature, using VirtualBox shared folder directly for fastq data, annotation or output directorycan significantly reduce the performance compared to a native (Ubuntu) system or VirtualBox native system.

BRB-SeqTools

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After Ubuntu has been successfully installed on VirtualBox, users can be go back to download and use BRB-SeqTools program.